Exprimental Study on the Protective Action of rhGM-CSF for Acute Lung Injury

Abstract

Anti-cancer agents and radiation therapies result in various complications on respiratory systems like interstitial pneumonia, acute lung damage, bacterial pneumonia and pneumomycosis in clinical oncology. Recently it is being interested in variable rolls of GM-CSF to protect the lungs. We infuse the ethyl alcohol and lipopolysaccharide (LPS) to damage lung orally, and then inject GM-CSF subcutaneously at the same time and then observe the changes of lung tissue. As a result, the number of total leucocytes, neutrophil, macrophage, total protein content, MPO, MDA, the number of alveoli per unit square meter, parenchyma thickness in the case with a dosage of 5μg/kg and 10μg/kg are decreased significantly than normal and control group. Settings and Design: We have infused 40% ethyl alcohol and LPS with a dose of 1.0mL/100g for 7 days orally to make a model of acute lung injury. And then we have injected with an rhGM-CSF dosage of 5μg/kg subcutaneously before infusion of alcohol and LPS so as to define the protective effectiveness from acute lung injury of GM-CSF. Results: The number of total leucocytes, neutrophil, macrophage, total protein content, MPO, MDA, the number of alveoli per unit square meter, parenchyma thickness in the case with a dosage of 5μg/kg and 10μg/kg are decreased significantly than normal and control group. Conclusions: We found out the protective action of acute lung injury of GM-CSF. Rational dosage of GM-CSF is 5μg/kg, and the number of total leucocytes, neutrophil and macrophage in the case with a dosage of 5μg/kg and 10μg/kg are increased significantly rather than the control group.

Keywords: GM-CSF, lipopolysaccharide (LPS), protective action, pneumomycosis, lipopolysaccharide, parenchyma

Cite this Article

Hyon-Min Ryang, Hye-Yong Nam. Experimental Study on the Protective Action of rhGM-CSF for Acute Lung Injury. Research & Reviews: Journal of Medical Science and Technology. 2019; 8(3): 4–8p.