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Antibacterial Activities of Medicinal Plant Leaves Extract Against Isolated Escherichia coli Bacteria from Different Water Sources
Abstract
Objective: Escherichia coli are the most common pathogenic bacteria to accountable for food and waterborne illness. Therefore, in this way the study was conducted to resolve the occurrence of E. coli bacteria from different water sources in around Tamil Nadu along with to determine their antibiotic resistance profiles. Methods: Water samples were analysed for the presence of E. coli bacteria using specific agar media Eosin Methylene Blue and McConkeys. Colony morphology, Gram staining, various biochemical tests and antibacterial tests performed by disc diffusion method were used to confirm E. coli bacteria. Results: The isolated bacteria were susceptible to Gentamycin (Mullai & Dindigul 18mm) and Tetracycline (Mullai 16mm &Vaigai 18mm) at 37°C for 24 hours. The isolate was susceptible to acetone leaves extract of Abutilon indicum, Calotropis gigantean and Solanum trilobatum in various concentrations (50µl, 75µl, & 100µl) and of A. indicum was most effective inhibition of zone formed in Mullai 24mm, Vaigai 18mm and Dindigul 23mm, triplicate value respectively. Conclusions: In this study revealed that E. coli bacteria were resistant to some antibiotics drugs. Compared to chemical antibiotic, the acetone leaves extract of A. indicum showed excellent inhibition effect (Mullai (24mm), and Dindigul drinking water (23mm) in 100µl concentrations only. But S. trilobatum was showed equal inhibition effect against Vaigai river water (18mm). Therefore, E. coli bacterium was controlled by natural antibiotics of A. indicum and it may reduce the resistance power of food and waterborne illness.
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References
Gratus, C., Wilson, S., Greenfield, S.M., Damery, S.L., Warmington, S.A., Grieve, R., Steven, N.M. and Routledge, P., 2009. The use of herbal medicines by people with cancer: a qualitative study. BMC Complementary and Alternative Medicine, 9(1), pp.1-7.
WHO traditional medicine strategy, Geneva: WHO; 2018.
Thirumurugan, K., Shihabudeen, M.S. and Hansi, P.D., 2010. Antimicrobial activity and phytochemical analysis of selected Indian folk medicinal plants. steroids, 1(7), pp.430-34.
Chew, A.L., Jessica, J.J.A. and Sasidharan, S., 2012. Antioxidant and antibacterial activity of different parts of Leucas aspera. Asian Pacific Journal of Tropical Biomedicine, 2(3), pp.176-180.
Richard, F.T., Joshua, A.T. and Philips, A.J., 2013. Effect of aqueous extract of leaf and bark of guava (Psidium guajava) on fungi Microsporum gypseum and Trichophyton mentagrophytes, and bacteria Staphylococcus aureus and Staphylococcus epidermidis. Advancement in Medicinal Plant Research, 1(2), pp.45-48.
Sarmiento, W.C., Maramba, C.C. and Gonzales, M.L.M., 2011. An in-vitro study on the antibacterial effect of neem (Azadirachta indica) leaf extract on methicillin-sensitive and Methicillin-resistant Staphylococcus aureus. PIDSP J, 12(1), pp.40-45.
Kalimuthu, K., Vijayakumar, S. and Senthilkumar, R., 2010. Antimicrobial activity of the biodiesel plant, Jatropha curcas L. International Journal of Pharma and Bio Sciences, 1(3), pp.1-5.
Aliero, A.A. and Wara, S.H., 2009. Validating the medicinal potential of Leptadenia hastata. African Journal of Pharmacy and Pharmacology, 3(6), pp.235-238.
MANTHABE, M., Nikolova, R.V., Lall, N. and Nyazema, N.Z., 2006. Antibacterial activities of medicinal plants used for the treatment of diarrhoea in Limpopo Province, South Africa. Journal of Ethnopharmacology, 105(1-2), pp.286-293.
Mittal J. Tinosporacordifolia: a multipurpose medicinal plant- A review. Journal of Medicinal Plants Studies, 2014: (2):32-47.
Gyles C. The growing problem of antimicrobial resistance.Can Vet J, 2011; 52 (8), pp. 817-820.
Sagar, K., Bashir, Y., Phukan, M.M. and Konwar, B.K., 2013. Isolation of lipolytic bacteria from waste contaminated soil: A study with regard to process optimization for lipase. Int. J. Sci. Technol. Res, 2(10), pp.214-218.
Seidler, R.J. and Starr, M.P., 1969. Factors affecting the intracellular parasitic growth of Bdellovibrio bacteriovorus developing within Escherichia coli. Journal of Bacteriology, 97(2), pp.912-923.
AbouzeedYM, ElfahemA, Zgheel F, Ahmed MO. Antibacterial in-vitro activities of selected medicinal plants against methicillin resistant Staphylococcus aureus from Libyan environment. J Environ Anal Toxicol 2013; 3: 194.
El-Hefny, M., Ashmawy, N.A., Salem, M.Z. and Salem, A.Z., 2017. Antibacterial activities of the phytochemicals-characterized extracts of Callistemon viminalis, Eucalyptus camaldulensis and Conyza dioscoridis against the growth of some phytopathogenic bacteria. Microbial pathogenesis, 113, pp.348-356.
Bauer AW, Kirby WM, Sherris JC, Truck M. Antibiotic susceptibility testing by a standard single disc method. American Journal of Pathology. 1966; 45: 493-496.
Odonkor ST and Ampofo JK. Escherichia Coli as an Indicator of Bacteriological Quality of Water: An Overview. Microbiology Research. 2013: Vol. 4 No. 1.DOI: 10.4081/mr.2013.e2.
Adzitey F, Haruna A. Antibiotic Susceptibility of Escherichia coli isolated from some Drinking Water Sources in Tamale Metropolis of Ghana. Current Research in Bacteriology. 2015: Volume 8 (2): 34-40.
Ahmed KKM, Rana AC, Dixit VK. Calotropis species (Ascelpediaceae): A comprehensive review. Pharmacog. Maga, 2005; 1(1), 4852.
Singh D, Gupta RS. Modulatory Influence of Abutilon indicum Leaves on Hepatic Antioxidant Status and Lipid Peroxidation against Alcohol-Induced Liver Damage in Rats. Pharmacology online. 2008, 1: 253-262.
Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K. Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitol Res. 2008; 102(5): 981-8.
Chandrashekhar VM, Nagappa AN, Channesh TS, Habbu PV. RaoKP. Antidiarrhoeal activity of Abutilon indicum Linn leaf extract. J Natural Remedies, 2004; 1(4): 12-6.
Roshan S, Ali S, Khan A, Tazneem B,Purohit MG. Wound Healing activity of Abutilon Indicum. Phcog Mag, 2008; 4, 85-88.
Bhajipale S.N. Evaluation of Anti-Arthritic Activity of Methanolic Extract of Abutilon Indicum. International Journal of ayurvedic and herbal medicine, 2012; 2(3): 598-603.
Famuyide IM, Aro OA 1, Fasina OF, Eloff NF, McGaw JL. Antibacterial activity and mode of action of acetone crude leaf extracts of under-investigated Syzygium and Eugenia (Myrtaceae) species on multidrug resistant porcine diarrhea genic Escherichia coli.BMC Vet Res. 2019; 15: 162.
Alia M, Haque S. A comparative study of in vitro antimicrobial, antioxidant and cytotoxic activity of Albizialebbeck and Acacia nilotica stem bark Bulletin of Faculty of Pharmacy, Cairo University.2018; Volume 56, Issue 1, P 34-38.
Nicoline F, Tanih, Roland N, Ndip. Evaluation of the Acetone and Aqueous Extracts of Mature Stem Bark of Sclerocaryabirrea for Antioxidant and Antimicrobial Properties Evidence-Based Complementary and Alternative Medicine, 2012; https://doi.org/10.1155/2012/834156.
Pandurangan A, Khosa RL, Hemalatha S. et al. Evaluation of Anti-inflammatory and Analgesic Activity of Root Extract of Solanum Trilobatum Linn J. Pharma. Res. 2008; 7(3):217–221.
Krishnan, K., Ramalingam, R.T, Venkatesan, G. Solanum trilobatum (Solanaceae) - an overview J. App. Bio. Sci. 2008; 2(3):109–112.
Franz E, Veenman C, Hoek A, Husman A, Blaak H. Pathogenic Escherichia coli producing Extended-Spectrum beta-Lactamases isolated from surface water and wastewater, Scientific Reports, 2015; vol. 5, no. 1, ID 14372.
DOI: https://doi.org/10.37591/rrjomv.v11i3.2585