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Arsenic Induced Cytotoxicity Study Against Human Epithelial Carcinoma (HeLa) Cell

Md. Shariful Islam, Alam Khan, Hrithita Aftab, Yoshifumi Kusumoto

Abstract


The present investigation was undertaken to find out the cytotoxic effects of various amounts of arsenic exposure to human epithelial carcinoma (HeLa) cell. Cells were treated with a final concentration (0, 10, 20, 30, 40 and 50 µg/l) of arsenite in 5 ml MEM for 24 h where 0 concentration was considered as control. Cell viability was determined by the trypan blue (TB) exclusion test using hematocytometer and automated BIO-RAD TC 10TM cell counting machine. It was demonstrated that survival rate of cancer cells treated with arsenite (0, 10, 20, 30, 40 and 50 µg/l) for 24 h decreased in a dose-dependent manner. Further, it was revealed that arsenite caused a time-dependent increase in cell death. In the highest concentration of arsenite (50 µg/l) cell survival rate was 1% and 2% in manual and automated counting. The possible mechanism that promote cytotoxicity of arsenite was addressed as oxidative damage of cells.

 

Keywords: Arsenite, HeLa Cells, Toxicity, Cell viability, Trypan blue assay, Hematocytometer

Cite this Article

Islam S, Khan A,Aftab H, Kusumoto Y. Arsenic Induced Cytotoxicity Study against Human Epithelial Carcinoma (HeLa) Cell. Research and Reviews: A Journal of Toxicology (RRJoT). 2015; 5(1): 1–8p.


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