Thymoquinone Induces Epigenetic Modulation in ROS Dependent Manner

Authors

  • Saad Mustafa
  • Jogendra Singh Pawar
  • Ilora Ghosh

DOI:

https://doi.org/10.37591/rrjot.v7i1.1323

Abstract

Traditional drugs have the ability to treat broad range of diseases by modulating the signaling pathwaysof inflammatory diseases and cancer. Epigenetic modulation is also one of the mechanisms already established as a process of action. Histone deacetylase (HDAC) inhibition leads to changes in gene expression promoting cell death. Thymoquinone (TQ), a bioactive compound from Nigella sativa seeds has shown to have an antineoplastic effect in many cancers. Here we have investigated HDAC inhibiting potential of TQ. It was observed that (TQ) inhibits the cell viability of HeLa and HepG2 cell lines in concentration dependent manner. It isable to enhanced the total oxidative species in both the cell lines. TQ treatment also causes a loss in mitochondrial membrane potential. The analysis of protein expression of HDAC1 and has shown a decrease in the level of HDAC1 and increase in the p21expressionthat confirming its ability to induce epigenetic modulation.

 

Keywords: Thymoquinone, Epigenetic, ROS, Mitochondrial membrane potential

 

Cite this Article

Saad Mustafa, Jogendra Singh Pawar, Ilora Ghosh. Thymoquinone Induces Epigenetic Modulation in ROS Dependent Manner. Research and Reviews: Journal of Toxicology. 2017; 7(1): 32–39p.

Downloads

Published

2019-04-09

Issue

Vol. 7 No. 1 (2017)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.