Induction of G1 Phase Cell Cycle Arrest and Apoptosis in Breast Cancer MCF-7 Cells by Sulphonamide Derivative CID-6861424

Abstract

Breast cancer is most frequently detected and leading cause of cancer death in women worldwide. 80% of the breast cancer are estrogen receptor positive and the presently available drugs are ineffective either due to intrinsic resistance or due to acquired resistance. Sulfonamide compounds are a class of compounds showing activities like, antibacterial, antiviral including antitumor. In the present study we have investigated the anti-cancerous activity of sulfonamide derivative CID-6861424 on breast cancer cell line, MCF-7. Our data shows inhibition of MCF-7 cell viability by CID-6861424 in a concentration and time dependent manner. 50μM was the IC₅₀ value of CID-6861424 on MCF-7 cell. The compound downregulated cyclin D1 and CDK 4/6, induced G₁ phase cell cycle arrest and apoptotic cell death. 50μM CID-6861424 upregulated ROS generation, disrupted mitochondrial membrane potential (Δψm), increased DNA damage and upregulated tumor suppressor protein, p53 in MCF-7 cells. The compound reduced phosphorylation of Akt and GSK-3β, downregulated Bcl-2 and upregulated Bax indicating apoptotic cell death. These results suggest CID-6861424 as a potential anticancer agent against breast cancer.

Keywords: CID-6861424; MCF-7 cell, cell cycle, double-strand DNA break, apoptosis.

Cite this Article

Sumit Kumar Gautam, Afreen Inam, Amir Azam, Neelima Mondal. Induction of G1 phase cell cycle arrest and apoptosis in breast cancer MCF-7 cells by sulphonamide derivative CID-6861424. Research & Reviews: A Journal of Toxicology. 2019; 9(2): 1–19p.