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Standardization of Barg-e-neem (Azadirachta indica)

Samreen Khan, Yasmeen Shamsi, Umar Jahangir, Mukesh Manjhi, Hina Fatima


The leaves of Azadirachta indica are commonly used medicinally in number of herbal products and herbal medicines The objective of the current study is to standardise Azadirachta indica leaves through qualitative and quantitative evaluation of various extracts. Physio-chemical analysis like determination of the pH value, determination of loss on drying, total ash value, acid insoluble ash value, different extractive values and presence of heavy metal was done on powdered leaves of the drug. The readings of the test sample were discovered to be within the suggested normal range. The refractive values of Thin layer chromatography of Azadirachta indica leaves alcoholic & hydroalcoholic extract also matched with control samples. The leaves of Azadirachta indica which were obtained from local market of khari baoli in Delhi are up to the mark and matched well with control values for the drug. Macroscopic evaluation of neem leaves was performed physically and the physical characteristics of sample found to match standard characters of Azadirachta indica plant.

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Joshi VK, Joshi A, Dhiman KS. The Ayurvedic Pharmacopoeia of India, development and perspectives. Journal of ethnopharmacology. 2017 Feb 2;197:32-8.

Sastri BN. The Wealth of India. A Dictionary of Indian Raw Materials and Industrial Products. Raw Materials, Vol. 6: LM. The Wealth of India. A Dictionary of Indian Raw Materials and Industrial Products. Raw Materials, Vol. 6: LM. 1962.

Kirtikar KR, Basu BD. Indian medicinal plants. Indian Medicinal Plants.. 1918..

Lokanatha O, Mamatha S, Reddy D. Antimicrobial activity of Azadirachta Indica (neem) leaf, bark and seed extracts. International Journal of Research in Phytochemistry and Pharmacology. 2013 Feb 28;3(1):1-4.

Mondall, N. K., et al. "Antifungal activities and chemical characterization of Neem leaf extracts on the growth of some selected fungal species in vitro culture medium." (2009).Vol. 13, pp. 49-53.

Shrivastava DK, Swarnkar K. Antifungal activity of leaf extract of neem (Azadirachta indica Linn). International Journal of current microbiology and Applied sciences. 2014;3(5):305-8.

Ezeonu CS, Imo C, Agwaranze DI, Iruka A, Joseph A. Antifungal effect of aqueous and ethanolic extracts of neem leaves, stem bark and seeds on fungal rot diseases of yam and cocoyam. Chemical and Biological Technologies in Agriculture. 2018 Dec;5:1-9.

Sanjoy B, Kim L, Mohamed S, Khatoon H, Yusoff FM. Antibacterial activity of neem (Azadirachta indica) leaves on Vibrio spp. isolated from cultured shrimp. Asian Journal of Animal and Veterinary Advances. 2013;8(2):355-61.

Grover A, Bhandari BS, Rai N. Antimicrobial activity of medicinal plants-Azadirachta indica A. Juss, Allium cepa L. and Aloe vera L. Int J Pharm Tech Res. 2011 Jun;3(2):1059-65.

Mistry KS, Sanghvi Z, Parmar G, Shah S. The antimicrobial activity of Azadirachta indica, Mimusops elengi, Tinospora cardifolia, Ocimum sanctum and 2% chlorhexidine gluconate on common endodontic pathogens: An in vitro study. European journal of dentistry. 2014 Apr;8(02):172-7.

Badam L, Joshi SP, Bedekar SS. 'In vitro'antiviral activity of neem (Azadirachta indica. A. Juss) leaf extract against group B coxsackieviruses. The Journal of communicable diseases. 1999 Jun 1;31(2):79-90.

Mosaddek AS, Rashid MM. A comparative study of the anti-inflammatory effect of aqueous extract of neem leaf and dexamethasone. ||| Bangladesh Journal of Pharmacology|||. 2008 May 6;3(1):44-7.

Kaur G, Sarwar Alam M, Athar M. Nimbidin suppresses functions of macrophages and neutrophils: relevance to its antiinflammatory mechanisms. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2004 May;18(5):419-24.

Sithisarn P, Supabphol R, Gritsanapan W. Antioxidant activity of Siamese neem tree (VP1209). Journal of Ethnopharmacology. 2005 May 13;99(1):109-12.

Ghimeray AK, Jin CW, Ghimire BK, Cho DH. Antioxidant activity and quantitative estimation of azadirachtin and nimbin in Azadirachta indica A. Juss grown in foothills of Nepal. African Journal of Biotechnology. 2009;8(13).

Nahak G, Sahu RK. Evaluation of antioxidant activity of flower and seed oil of Azadirachta indica A. juss. Journal of Applied and Natural Science. 2011 Jun 1;3(1):78-81.

Bhat M, Kothiwale SK, Tirmale AR, Bhargava SY, Joshi BN. Antidiabetic properties of Azardiracta indica and Bougainvillea spectabilis: in vivo studies in murine diabetes model. Evidence-Based Complementary and Alternative Medicine. 2011 Jan 1;2011.

Akter R, Mahabub-Uz-Zaman M, Rahman MS, Khatun MA, Abdullah AM, Ahmed NU, Islam F. Comparative studies on antidiabetic effect with phytochemical screening of Azadirachta indicia and Andrographis paniculata. IOSR Journal of Pharmacy and Biological Sciences. 2013 Jan 9;5(2):122-8.

Antimalarial effect of Neem leaf and Neem stem bark extracts on plasmodium berghei infected in the pathology and treatment of malaria. Akin-Osanaiye, B C, et al. 1, 2013, International Journal of Research in Biochemistry and Biophysics, Vol. 3, pp. 7-14.

An antimalarial neem leaf extract has both schizonticidal and gametocytocidal activities. Udeinya, J I, et al. 2, 2008, American Journal of Therapeutics, Vol. 15, pp. 108-110.

Immunomodulatory and growth promoting effects of neem leaves infusion in broiler chicks. Durrani, E R, et al. 2008, Sarhad Journal of Agriculture, Vol. 24, pp. 316-321.

“Immunopotentiating effects of Azadirachta indica (Neem) dry leaves powder in broilers, naturally infected with IBD virus. Sadekar, R D, et al. 11, 1998, Indian Journal of Experimental Biology, Vol. 36, pp. 1151-53.

Evaluation of the wound healing activity of methanolic extract of Azadirachta Indica (Neem) and Tinospora cordifolia (Guduchi) in rats. Barua, C C, et al. 2010, Pharmacologyonline, Vol. 1, pp. 70-77.

The wound healing effects of aqueous leave extracts of azadirachta indica on wistar rats. Emeka, A Osunwoke, et al. 6, 2013, Journal of Natural Science and Research,, Vol. 3, pp. 181-86.

Nimbolide a limonoid from Azadirachta indica inhibits proliferation and induces apoptosis of human choriocarcinoma (BeWo) cells. Kumar, G Harish, et al. 3, 2009, Investigational New Drugs, Vol. 27, pp. 246–252.

The neem limonoids azadirachtinThe neem limonoids azadirachtin in an animal model of oral oncogenesis. Kumar, G H, et al. 4, 2010, Investigational New Drugs , Vol. 28, pp. 392-401.

Neem leaf extract inhibits mammary carcinogenesis by altering cell proliferation, apoptosis, and angiogenesis. Arumugam, A, et al. 1, 2104, Cancer Biology and Therapy, Vol. 15, pp. 26-34.

To evaluate the antigingivitis and antipalque effect of an Azadirachta indica (neem) mouthrinse on plaque induced gingivitis: a double blind, randomized, controlled trial. Chatterjee, A, et al. 4, 2011, Journal of Indian Society of Periodontology, Vol. 15, pp. 398-401.

The inhibiting effect of Azadirachta indica against dental pathogens. Lekshmi, N C J P, et al. 1, 2012, Asian Journal of Plant Science and Research, Vol. 2, pp. 6-10.

Hepatoprotective activity of the neem-based constituent azadirachtin-A in carbon tetrachloride intoxicated Wistar rats. Baligar, N S, et al. 4, 2014, Canadian Journal of Physiology and Pharmacology, Vol. 92, pp. 267-77.

Effect of Azadirachta indica (neem) leaf aqueous extract on paracetamol induced liver damage in rats. Bhanwra, S. 1, 2000, Indian Journal of Physiology and Pharmacology, Vol. 44, pp. 64-68.

Gastroprotective effect of aqueous extract of neem Azadirachta indica on induced gastric lesion in rats. Ofusori, D A, et al. 2010, International Journal of Biological and Medical Research, Vol.1, pp. 219-22.

Azadirachta indica attenuates cisplatin induced neurotoxicity in rats. Moneim, A E A. 3, 2014, Indian Journal of Pharmacology, Vol. 46, pp. 316-321.

Azadirachta indica attenuates cisplatin-induced nephrotoxicity and oxidative stress. Moneim, A E A, Othman, M S and Aref, A M. 2014, BioMed Research International, Vol. 2014, pp. 1-11.

Wound healing Potential of Mellia azedarach L. leaves in Alloxan induced Diabetic Rats. Vidya, V, Srinivasan, D and Sengottuvelu, S. 7, 2012, Global Journal of Research on Medicinal Plants & Indeginous Medicine, Vol. 1, pp. 265-71.

WHO. Quality control methods for medicinal plant material. Geneva : s.n., 1998.

Thin Layer Chromatography: A Tool of Biotechnology for Isolation of Bioactive Thin Layer Chromatography: A Tool of Biotechnology for Isolation of Bioactive. Sanjeet Kumar, K. Jyotirmayee, Monalisa Sarangi. 1, 2013, Int. J. Pharm. Sci, Vol. 18, pp. 126-132.

A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Azwanida, N N. 2015, Medicinal & Aromatic Plants.


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