Production of Single-domain Antibodies Library by Phage Display Technology

Authors

  • Ajit Singh Professor (Immunology), Department of Veterinary Microbiology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, Haryana India
  • Neelam Pandey
  • Pooja Jangra
  • Jagveer Rawat

DOI:

https://doi.org/10.37591/rrjoi.v2i3.1168

Abstract

Single-domain antibodies (dAbs) are synthesized from variable domain gene-segments of atypical camelid or shark “heavy chain antibodies” (HCAbs) by phage display technology. We have constructed a phage display library of > 5 × 107 dAb clones derived from HCAb genes in B lymphocyte repertoire of a lipopolysaccharide (LPS)-immunized Indian desert camel and isolated dAb-phage clones by panning of the library against LPS and Staphylococcus aureus exotoxins. Nucleotide and deduced amino-acid sequence of the dAbs had key features of camelid HCAb variable domain. Soluble dAbs were of ~16 kDa size, thermostable, Ag-specific and toxin-neutralizing in vitro. Their therapeutic and diagnostic potential is being explored.

 

Keywords: Single-domain antibodies, library, phage display

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Published

2019-04-02

Issue

Vol. 2 No. 3 (2012)

Section

Research Article

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