Cell Membrane Immunogens of a Biofilm-producing Staphylococcus aureus Isolate Adjuvanted with Bacterial Chromosomal DNA Are Protective in a Mouse Mastitis Model

Authors

  • Krishan Sharma
  • Ajit Singh Professor (Immunology), Department of Veterinary Microbiology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, Haryana India

DOI:

https://doi.org/10.37591/rrjoi.v3i3.1182

Abstract

Biofilm-forming staphylococci have been implicated in persistent bovine udder infections. Prophylactic potential of Staphylococcus aureus biofilm phenotype-associated immunogens in mouse models of systemic, but not intramammary infections has been demonstrated. In the present study, the authors observed protection in a murine model of intramammary infection by two subcutaneous injections at four-week interval of biofilm-forming S. aureus cell membrane antigens mixed with homologous chromosomal DNA as adjuvant, when challenged after eight weeks by intramammary route with 106 CFUs of biofilm-forming S. aureus. The immunoblot analysis indicated that five biofilm phenotype-associated polypeptides, 105, 90, 46, 36, and 33 kDa were more important than others as immunogens. These findings would be useful in selection of biofilm phenotype-associated S. aureus immunogen(s) for a candidate vaccine against bovine mastitis.

 

Keywords: Staphylococcus aureus, biofilms, cell membrane antigens, chromosomal DNA adjuvant, murine mastitis model

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Published

2019-04-02

Issue

Vol. 3 No. 3 (2013)

Section

Research Article

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