Self-Evaluation of CO2 Incubator before In-Vitro Handling of Gametes and Embryos
Abstract
Abstract
ART (Assisted Reproductive Technologies) is known as the technologies/techniques used for the treatment of Infertility. ART involves different types of newly and advanced techniques such as ICSI (Intra-Cytoplasmic Sperm Injection), MESA (Microsurgical Epididymis Sperm Aspiration), PESA (Percutaneous Epididymal Sperm Aspiration),TESA (Testicular Sperm Aspiration), IMSI (Intra Morphologically selected sperm injection), GIFT (Gamete Intra-fallopian Transfer), ZIFT (Zygote Intra-fallopian Transfer) and Assisted hatching. The self-evaluation and maintenance of all equipment used in the ART is heavily important for the better outcomes. As ART is referred as result oriented field of the medical sciences, so it is very important to care the all types of equipment used for the treatment in a very delegate way. Human gametes and Embryos are cultured in a Co2, O2, pH and Temperature based equipment called Incubator. The purpose of this article is to highlight the importance of the maintenance of Incubator used for the handling of In-Vitro products for the better outcome.
Keywords: Gametes; Embryos; Aspiration; Cytoplasmic; pH.
Cite this Article
Saurabh, Sanjiv Kalia. Self Evaluation of CO2 Incubator Before In-Vitro Handling of Gametes and Embryos. Research & Reviews: Journal of Medical Science and Technology. 2020; 9(2): 7–11p.
References
Boone WR, Shapiro SS. Quality control in the in vitro fertilization laboratory. Theriogenology 1990;33:23–50.
Rhodes TL, McCoy TP, Higdon HL III, Boone WR. Factors affecting assisted reproductive technology (ART) pregnancy rates: a multivariate analysis. J Assist Reprod Genet 2005;22:335–46.
Fertil Steril, Definitions of infertility and recurrent pregnancy loss: a committee opinion, 2013 Jan;99(1):63. doi: 10.1016/j.fertnstert.2012.09.023. Epub 2012 Oct 22..
Swain J.E. Is there an optimal pH for culture media used in clinical IVF?. Hum. Reprod. Update. 2012; 18: 333-339.
Jason E. Swain Decisions for the IVF laboratory: comparative analysis of embryo culture incubators, Volume 28, Issue 5, P535-547.
Basler, A. (1978), timing of meiotic stage in oocyte of the Syrian hamster (mesocricetus auratus) and analysis of induced chromosome aberrations. Hum. Genet. 42, 67-77.
Sun X.F., Wang W.H., Keefe D.L, Overheating is detrimental to meiotic spindles within in vitro matured human oocytes., Zygote. 2004; 12: 65-70.
Wang W.H., Meng L., Hackett R.J., Odenbourg R., Keefe D.L., Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy., Hum. Reprod. 2001; 16: 2374-2378.
Leese H.J., Baumann C.G., Brison D.R., McEvoy T.G., Sturmey R.G., Metabolism of the viable mammalian embryo: quietness revisited., Mol. Hum., Reprod. 2008; 14: 667-672.
Stoddart N., Rho H., Maisel H., Zhao T., Tarantino S., Fleming S., Influence of the position of the culture dish within the incubator chamber and temperature of culture media on clinical outcome following Assisted Reproductive Technology (ART) treatment., Fertil. Steril. 2003; 80: s164-s165.
Basler, A. (1978), timing of meiotic stage in oocyte of the Syrian hamster (mesocricetus auratus) and analysis of induced chromosome aberrations. Hum. Genet. 42, 67-77.
Whitten WK. Culture of tubal mouse ova. Nature 1956;176:96.
Chan AW, Luetjens CM, Dominko T, Ramalho-Santos J, Simerly CR, Hewitson L, Schatten G., TransgenICSI reviewed: foreign DNA transmission by intracytoplasmic sperm injection in rhesus monkey, Mol Reprod Dev. 2000 Jun;56(2 Suppl):325-8.
Borkow, G., Gabbay, J., 2004. Putting copper into action: copper- impregnated products with potent biocidal activities. FASEB, J. 18, 1728–1730
Spaulding EH. Chemical disinfection of medical and surgical materials. In: Lawrence C, Block SS, eds. Disinfection, sterilization, and preservation. Philadelphia: Lea & Febiger, 1968:517-31.
Cooke S., Tyler J.P., Driscoll G., Objective assessments of temperature maintenance using in vitro culture techniques., J. Assist. Reprod. Genet. 2002; 19: 368-375.
Fujiwara M., Takahashi K., Izuno M., Duan Y.R., Kazono M., Kimura F., Noda Y., Effect of micro-environment maintenance on embryo culture after in-vitro fertilization: comparison of top-load mini incubator and conventional front-load incubator., J. Assist. Reprod. Genet. 2007; 24: 5-9.
Kirkegaard K., Hindkjaer J.J., Grondahl M.L., Kesmodel U.S., Ingerslev H.J., A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator., J. Assist. Reprod., Genet. 2012; 29: 565-572.
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