![Open Access](https://medicaljournals.stmjournals.in/lib/pkp/templates/images/icons/fulltext_open_medium.gif)
![Restricted Access](https://medicaljournals.stmjournals.in/lib/pkp/templates/images/icons/fulltext_restricted_medium.gif)
Isolation of Lactobacillus Acidophilus with A High Acid-Producing Capacity and Its Common Characteristics
Abstract
Some strains of lactobacilli like spore Lactobacillus offer good merits in normalizing intestinal micro biota, resisting selectively to intestinal pathogens, exerting resistance to heat, acid and antibiotics, and are of good storage stability. This research work has been conducted in order to isolate a certain strain of Lactobacillus that offers high resistance to the gastric acid, bile and pathogens, and mutualistic symbiosis with useful intestinal microbes, and identifying its characteristics of actions. A strain of lactobaillus isolated from several sources was investigated in terms of its ability of lactic acid production and identified by examining the survival rate in self-made gastric juice and bile, and the ability of resisting to antibiotics, and symbiosis with useful intestinal microbes. Lactobacillus Acidophilus, isolated and identified, offers good resistance to self-made gastric juice and self-made bile, good antibacterial properties against pathogens, and good symbiosis with useful intestinal microbes. In the mixed culture of the isolated L. Acidophilus and Bifidobacterium bifidum, the number of these two species of bacteria increased significantly after 24 h rather than the single culture. The size of the inhibitory zone of the lactobacilli for E. coli, Shigelladysenteriae and paratyphoid bacillus was 15.3, 16.8, and 16.2 mm respectively. The survival rate of the isolated strain was 93.4% in the self-made gastric juice after 3 h whereas it was 88.0% in the 2% bile. It has good prospects of being widely used in prevention and treatment of intestinal infections.
Keywords
References
Natividad J, Verdu E: Modulation of intestinal barrier by intestinal microbiota: pathological and therapeutic implications. Pharmacological Res 2013, 69 (1): 42–51.
Silva HD, Millard P, Soper N, Kettlewell M, Mortensen N, Jewell D: Effects of the faecal stream and stasis on the ileal pouch mucosa. Gut 1991, 32 (10): 1166–1169.
Famularo G, Moretti S, Marcellini S, De Simone C: Stimulation of immunity by probiotics. In: Fuller R, ed. Probiotics: therapeutic and other beneficial effects. London: Chapman and Hall, 1997, 13361.
Bermudez-Brito M, Plaza-Diaz J, Munoz-Quezada S, Gomez-Llorente C, Gil A: Probiotic mechanisms of action. Ann Nutr Metab 2012, 61: 160–174.
Plaza-Diaz J, Fernandez-Caballero JA, Chueca N, Garcia F, Gomez-Llorente C, Saez-Lara MJ, Fontana L, Gil A: Pyrosequencing analysis reveals changes in ntestinal microbiota of healthy adults who received a daily dose of immunomodulatory probiotic strains. Nutrients 2015, 7: 3999–4015.
Plaza-Diaz J, Gomez-Llorente C, Abadia-Molina F, Saez-Lara MJ, Campana-Martin L, Munoz-Quezada S, Romero F, Gil A, Fontana L: Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on hepatic steatosis in Zucker rats. PLoS ONE 2014, 9.
Fuller R: Probiotics in man and animals. J Appl Bacteriol 1989, 66: 365–378.
Abdelouhab K, Rafa H, Toumi R, Bouaziz S, Medjeber O, Touil-Boukoffa C: Mucosal intestinal alteration in experimental colitis correlates with nitric oxide production by peritoneal macrophages: Effect of probiotics and prebiotics. Immunopharmacol Immunotoxicol 2012, 34: 590–597.
Toumi R, Abdelouhab K, Rafa H, Soufli I, Raissi-Kerboua D, Djeraba Z, Touil-Boukoffa C: Beneficial role of the probiotic mixture Ultra biotique on maintaining the integrity of intestinal mucosal barrier in DSS-induced experimental colitis. Immunopharmacol Immunotoxicol 2013, 35: 403–409.
Itoh, T., Fujimoto, Y., Kawai, Y., Toba, T., & Saito, T. (1995). Inhibition of food-borne pathogenic bacteria by bacteriocins from Lactobacillus gasseri. Letters in Applied Microbiology, 21(3),
–141.
Brady L J, Gallher D and Busta F 1998 The role of probiotic cultures in the prevention of the colon cancer J Nutrition 41(1) 50–61
Markowitz J E and Bengmark S 2003 Probiotics in health and disease in the pediatric patient Pediatric Clin North Am 3(1) 49–54
Samarzua D 2009 Probiotic bacteria in prevention and treatment of diarrhea Mljekarstvo 59 (1)
–32
Cameron D 2017 Probiotics for gastrointestinal disorders proposed recommendations for children of the Asia Pacific region World J Gastroenterol 23(45) 7952–7964
Duggan C, Walker W A and Watkins J B 2003 Nutrition In Pediatrics 3rd Edition London BC Decker 3(1) 223–233
Kerrya R G 2018 Benefaction of probiotics for human health A review Journal of Food and Drug Analysis Volume 26 Issue 3 July 2018 Pages 927–939
Refbacks
- There are currently no refbacks.