Improvement of Motor and Sensory–Motor Behaviors after the Human Amniotic Epithelial Cells Transplantation in 6-OHDA Lesioned Corpus Striatum of Wistar Albino Rats

Authors

  • Ravisankar Periyasamy Department of Anatomy, Tagore Medical College & Hospital, Rathina Mangalam, Vandalur Post, Chennai -600048, Tamil Nadu, India
  • Ramesh Kumar Radhakrishnan Department of Anatomy, Dr. A.L.M.P.G.I.B.M.S, University of Madras Taramani, Chennai -113, Tamil Nadu, India
  • Muthusamy Rathinasamy Department of Anatomy, S.R.M Dental College-Deemed University, Ramapuram, Chennai, Tamil Nadu, India

DOI:

https://doi.org/10.37591/rrjon.v4i2.974

Abstract

Deficits in apomorphine-induced rotation and rotarod in rat have been proposed as a nondrug-induced model of the akinesia associated with the Parkinson’s disease. In the present study, we examined the disabilities in the rotarod and apomorphine-induced rotations have been observed after the 6-hydroxydopamine-induced denervation in the striatum. These impaired activities have reversed after the Human Amniotic Epithelial (HAE) cells transplantation during long-term observation. The neuronal densities and diameter were reduced in the 6-OHDA-infused striatum and the densities and diameter of the neurons were slightly increased after the HAE cells transplanted striatum. The HAE cells did not have any surface antigen to create the graft-versus-host disease.  HAE cells from term human placenta express surface markers and genes characteristic of embryonic stem cells and have the ability to differentiate into all three germ layers, including tissues of endodermal origin i.e., liver. HAE cells possess the ability to differentiate into cells with characteristics of functional hepatocytes, in vitro and in vivo, thus representing a useful and noncontroversial source of cells for transplantation. In the present investigation, the HAE cells transplanted animals showed improvement on the motor, sensorimotor and muscular coordination.

 

Keywords: Human amniotic epithelial cells, Apomorphine-induced rotation,
DiI labelling, Rotarod, 6-OHDA lesion

 

Downloads

Published

2019-03-26

Issue

Vol. 4 No. 2 (2014)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.