Decolorization of Diazo Dye Amido Black 10B by a Bacterium Kerstersia sp. Strain VKY1

Abstract

Amido Black 10B, a diazo dye having various industrial applications was decolorized by a bacterium Kerstersia sp. strain VKY1. The effect of various parameters such as dye concentration, pH, temperature and NaCl concentration was investigated. At 200 mg/l dye concentration, the strain decolorized 81% of the dye at temperature 35°C and pH 7.0 in 36 h of incubation. This strain decolorized the dye over a wide range of pH (5–11), temperature (25–55°C), and NaCl concentration (5–30 g/l). Further, the strain decolorized Amido Black 10B up to 600 mg/l in 48 h. The cell free extract of the strain VKY1 grown on Amido Black 10B exhibited the azoreductase activity of 0.7034 µM/min/mg protein. UV-Vis and HPLC investigation of the dye decolorized sample indicated the formation of breakdown products of azo bond.

Keywords: Azo dye, toxic, decolorization, Amido Black 10B, Kerstersia sp., azoreductase

Cite this Article

Masarbo Ramesh S, Reddy Pooja V, Karegoudar TB et al. Decolorization of Diazo Dye Amido Black 10B by a Bacterium Kerstersia sp. Strain VKY1. Research & Reviews: A Journal of Microbiology and Virology. 2018; 8(3):    37–43p.