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Population Genetics and Diversity of Human Adenovirus in Two Tertiary Hospitals in Southern Nigeria

Dirisu John, Agbakoba Nneka, Eki-Udoko Fidelis, Elo-Ilo Jacinta C., Oladipo Olukayode, Okwelogu Somadina

Abstract


The study of dynamic changes in genetic heterogeneity can shed light on the evolution of viruses because it is known that the genetic variety of viral populations within their hosts affects disease progression, treatment outcome, medication resistance, cell tropism, and transmission risk. Our study's primary goal is to identify trends in the genetic makeup and variety of human adenoviruses using samples taken from children who attend two distinct tertiary hospitals in Southern Nigeria's Benin City and Nnewi. In Benin City and Nnewi, 330 pediatric patients' samples from tertiary hospitals were gathered, and after DNA extraction and Sanger sequencing, the samples were examined. The obtained genetic information matrix was used to calculate the Jaccard similarity index, genetic distance, and genetic diversity studies. According to the Analysis of Molecular Variance (AMOVA), this variation was more evenly distributed within each site's population (97%) than it was between them (3%). The effective number of alleles Ne, which represents genetic diversity at the population level, was 2.358 for Benin City and 2.255 for Nnewi, respectively. This shows that each of the studied loci had an average of 2.358 and 2.255 alleles per locus in these two populations. As a result, Nnewi has less genetic diversity of human adenovirus than Benin.

Keywords


Genetic diversity, human adenovirus, AMOVA, loci, children, population

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DOI: https://doi.org/10.37591/rrjoi.v12i3.3020

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