Aluminum Oxide Nanoparticle-Adjuvanted Pasteurella multocida B:2 Vaccine is Potent and Efficacious and is Able with Keyhole Limpet Hemocyanin as a Marker to Differentiate Infected from Vaccinated Animals (DIVA)
DOI:
https://doi.org/10.37591/rrjoi.v5i2.1251Abstract
Pasteurella multocida B:2 causes hemorrhagic septicemia (HS) in bovines in Asia. For effective implementation of HS control program in India, a safe and efficacious vaccine to provide long lasting protection and allow differentiation of infected from vaccinated animals (DIVA) has to be developed. The objectives of the present study were to develop HS-DIVA vaccine with a companion immunodiagnostic test using keyhole limpet hemocyanin (KLH) as a marker, and to improve its potency and efficacy using aluminum oxide nanoparticles (Al2O3 NPs) as adjuvant in a mouse model. The experimental vaccine was constructed by mixing Al2O3 NPs and KLH with formalin-inactivated P. multocida B:2 P52 strain. Swiss albino mice (n = 8/group) were inoculated twice at three-week interval. Sixteen days later, the mice were challenged with 50 × lethal dose 50 of virulent P. multocida B:2 P52 strain and protection levels were determined for each group. Antibody levels against bacterial antigens and KLH were determined by indirect ELISA. The mice in Al2O3 NPs + KLH + bacterin [HSVac-I] group showed 100% protection. While, Al2O3 NPs + bacterin [HSVac-II], KLH + bacterin [HSVac-III], bacterin [HSVac-IV] and alum-precipitated bacterin [HSVac-V] groups had 87.5, 62.5, 62.5 and 12.5% protection, respectively. Anti-bacterial antibody levels in the HSVac -I, -II and -III groups were significantly higher than those in HSVac-IV (*P < 0.05) and HSVac-V (**P < 0.001) groups on 14, 21, 28 and 35 days post-immunization. Anti-KLH antibodies had high titres in all the KLH-positive groups and persisted as long as antibacterial antibodies. In conclusion, KLH was suitable as a marker for HS-DIVA vaccine and with Al2O3 NPs improved the HS vaccine potency and efficacy.
Keywords: Al2O3 nanoparticles, KLH marker antigen, bovine hemorrhagic septicemia, DIVA vaccine, Pasteurella multocida B:2
Cite this Article
Ray Susim Mukul, Singh Ajit. Aluminum oxide nanoparticle-adjuvanted Pasteurella Multocida B:2 vaccine is potent and efficacious and is able with keyhole limpet hemocyanin as a marker to differentiate infected from vaccinated animals (DIVA). Research and Reviews: Journal of Immunology (RRJoI). 2015; 5(2): 8–16p.
Downloads
Published
Issue
Section
License
Declaration and Copyright Transfer Form
(to be completed by authors)
I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.
- I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
- I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
- I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
- I/We also agree to the authorship of the article in the following order:
Author’s name
1. ________________
2. ________________
3. ________________
4. ________________
| We Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal. |
