Assessment of Micronuclei in the Cultured Peripheral Blood Lymphocytes of Human Volunteers Consuming Various Forms of Tobacco in Mizoram

Authors

  • Bawitlung Lalruatfela
  • Ganesh Chandra Jagetia

DOI:

https://doi.org/10.37591/rrjomst.v8i2.1627

Abstract

Tobacco consumption is highly prevalent in Mizoram—a northeastern state of India—and it is one of the main causes of high cancer incidence. Peripheral blood was collected from 245 volunteers within the age group of 18–67 years with their informed consent and micronuclei were prepared in cultured lymphocytes by blocking cytokinesis. Binucleated (BN) cells bearing micronuclei were scored blindly under a fluorescence microscope, where a total of 3000 BN cells were scored for each individual. Significance for micronuclei was determined by Mann-Whitney U test, whereas Pearson’s correlation coefficient was calculated to determine the correlation of micronuclei formation with age. Linear regression was performed to study the effect of different variables on the micronuclei frequency. Frequency of micronuclei increased significantly in individuals who regularly consumed tobacco in any form when compared to individuals of the control cohort. The individuals who were using smokeless tobacco in the form of tuibur (tobacco brew) had a higher frequency of micronuclei than the other form of tobacco. Our study indicated that the use of tobacco in any form is a risk factor for higher amount of DNA damage, which may be the cause of high cancer incidences in Mizoram.

 

Keywords: Human lymphocytes, DNA damage, micronuclei, tobacco, tuibur

 

Cite this Article

Bawitlung Lalruatfela, Ganesh Chandra Jagetia. Assessment of Micronuclei in the Cultured Peripheral Blood Lymphocytes of Human Volunteers Consuming Various Forms of Tobacco in Mizoram. Research & Reviews: Journal of Medical Science and Technology. 2019; 8(2): 17–26p.

Downloads

Published

2019-10-01

Issue

Vol. 8 No. 2 (2019)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.