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Influence of Reptin on acute pancreatitis model of the rat

Chol Kang, Hun Ryong, Song-Chol Mun, Gun-Son Ri


Background and Aim: In acute pancreatitis, hyperproduction of inflammatory mediators, including inflammatory cytokines, exacerbation of tissue injury due to microcirculatory disturbances, secondary activation of inflammatory cells in distant organs, and the cascade are the pathogenic basis of pancreatitis severity. Therefore, inhibition of pancreatic enzyme activity, blocking production, and preventing the activation of inflammatory cells is a practical way to prevent the development of severe acute pancreatitis and multiorgan failure. In our study, we sought to elucidate the effect of leptin on a rat model of severe acute pancreatitis. Methods: We prepared an acute pancreatitis model in rats by intraperitoneal injection of 20 μg/kg of leptin and single injection of 0.1 mL/100g of bile into a pancreatic duct extending from the duodenal papilla, measured survival, serum amylase activity, MPO activity in pancreatic tissue, SOD activity and MDA content, and observed the histopathological findings of pancreatic tissue. Results: Pre-administration of 20 μg/kg leptin improved the survival of rats, decreased serum amylase activity, MPO activity in pancreatic tissue, increased MDA content, increased SOD activity, and improved the histopathological features of pancreatic tissue.


Leptin, SAP

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Bakker O et al., Extrapancreatic necrosis without pancreatic parenchymal necrosis: a separate entity innecrotising pancreatitis? Gut 62, 1475-1480. 2013

Ding S P et al., A mouse model of severe acute pancreatitis induced with caerulein and lipopolysaccharide. World. J. Gastroenterol. 9(3), 584~589, 2014.

Frossard J et al., The role of intercellular adhesion molecule and neutrophils in acute pancreatitis and pancreatitis- associated lung injury. Gastroenterology. 116, 694-701, 2013.

Hackert T. et al., Platelete function in acute experimental pancreatitis induced by ischemia-repefusion. Br. J. Surg. 92(6), 724~728, 2005.

Inoue K et al., Further evidence for endothelin as an important madiator of pancreatic and intestinal ischemia in severe acute pancreatitis. Pancreas. 26(3), 218~223, 2008.

Laszlo Czako, et al., The pathogenesis of L-arginine-induced acitenecrotizing pancreatitis: Infla㎜atory mediators and endogenous cholecystokinin]. J. Physiol. 94, 43~50, 2011.

Leindler L et al., Importance of cytokines, nitric oxide, and apoptosis in the pathological process of necrotizing pancreatitis in rats. Pancreas. 29(2), 157~161, 2010.

Konturek PC, Jaworek J, Maniatoglou A, et al. Leptin modulates the inflammatory response in acute pancreatitis. Digestion. 2002, 65(3): 149-160.

La Cava A, Matarese G. The weight of leptin immunity. Nature reviews. Immunology, 2004, 4(5), 371-379.

Sanchez-Margalet V, Martin-Romero C, Santos-Alvarez J, et al. Role of leptin as an immunomodulator of blood mononuclear cells: mechanisms of action. Clin Exp Immunol. 2003, 133(1): 11-19.

Gonzalez A, Merino B, Marroqui L, et al. Insulin hypersecretion in islets from diet-induced hyperinsulinemic obese female mice is associated with several functional adaptations in individual beta-cells. Endocrinology, 2013, 154(10): 3515-3524.

Dossin O. Laboratory tests for diagnosis of gastrointestinal and pancreatic diseases. Top Comp Anim Med, 2011, 26(2): 86-97.


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