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Screening and Characterisation of Antibacterial Actinomycetes Isolated From Earthworm

Neethu M.C., Namitha Bhas, Anjana J.C.

Abstract


The actinomycetes are considered to be the most potent source for the production of secondary metabolites, antibiotics and other bioactive compounds. The present study dealt with the isolation of the actinomycetes from the earthworm, Eisenia fetida. The actinomycetes were identified using slide culture technique and various biochemical tests. The isolates (S1 and S2) showed activity against Gram positive and Gram negative bacteria in primary screening by perpendicular streak method. In primary screening the isolate S2 highly inhibited the growth of Pseudomonas sp. The antibacterial metabolites were extracted using the solvent ethyl acetate to the culture filtrate in the ratio (1:1). The secondary screening by agar well diffusion method was also performed against organisms like E. coli, Pseudomonas sp., Staphylococcus sp., Klebsiella sp. and Bacillus sp. The metabolites were further analyzed by TLC and FTIR techniques. The extract was evaluated for antioxidant activity using DPPH method with ascorbic acid as the standard. The extract show high antioxidant activity (65%) than ascorbic acid (52%). Thus the actinomycetes isolated from an alternative source should be further exploited to be pursued as sources of novel bioactive secondary metabolites.

 

Keywords: Actinomycetes, bioactive, earthworm, perpendicular streak, FTIR.

Cite this Article

Neethu M.C., Namitha Bhas, Anjana J.C. Screening and Characterisation of Antibacterial Actinomycetes Isolated From Earthworm. Research & Reviews: A Journal of Microbiology and Virology. 2019; 9(1): 77–84p.


Keywords


Actinomycetes, bioactive, earthworm, perpendicular streak, FTIR.

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References


Donia M., Humann MT. Marine natural products and their potential applications as anti infective agents. Lancet infect dis. 2003; 3: 338-348.

Bull AT, Stach JE. Marine actinobacteria: new opportunities for natural product search and discovery. Trends Microbiol. 2004; 15:491-9.

Sangliere JJ, Wellington EM, Behal V et al. Novel Bioactive compounds from actinomyces. Res. Microbiol. 1993; 144: 661-663.

Schatz A, Bugie E, Waksman SA. Streptomycin, a substance exhibiting antibiotic activity against gram-positive and gram-negative bacteria-1944. Clin Orthop Relat Res. 2005; 437: 3-6.

Bull AT, Stach, JM, Ward AC. Marine actinobacteria perspectives, challenges, future directions. Antonie van leeuwenhook. 2005; 87: 65-79.

Ishii. Y and Mihara H. Process for the production of dried earthworm powder and anti hyperlipemic, antidiabetic, antihypertensive and anti-hypotensive preparations containing dried earthworm powder as an active ingredient. U.S. Patent Documents. 1992; 1-38.

Williams ST and Davies FL. Use of antibiotics for selective isolation and enumeration of Actinomycetes in soil. Journal of General Microbiology 38:251-261 WHO 2002. Deaths by cause, sex and mortality stratum in WHO Regions, estimates for 2001.World Health Report, World Health Organization.1995.

Williams ST, Goodfellow M, Alderson G. Genus Streptomyces Waksman and Henrici 1943, 339AL. In: Williams ST, Sharpe ME, Holt JG, Editors. Bergey's Manual of Systematic Bacteriology, Vol. 4., Baltimore: Williams and Wilkins Co. 1989. p. 2452-2492.

Homans AL and Fuchs A. Direct bioautography on thin-layer chromatograms as a method for detecting fungitoxic substances. J. Chromatogr. 1970; 51: 327-329.

Holt JG, Krieg NR, Sneath PHA. Bergey’s manual of Determinative Bacteriology, 9thedn, pp 571-701, Williams & Wilkins, Baltimore. 1994.

James PD, Edwards C, Dawson M. The effects of temperature, pH and growth rate on secondary metabol- ism in Streptomyces thermoviolaceus grown in a chemostat. J. Gen. Microbiol. 1991; 137:1715-1720. [DOI]

Kokare, CR, Mahadik, KR, Kadam SS, Chopade BA. Isolation, characterization and antimicrobial activi- ty of marine halophilic Actinomonospora species AH1 from the West Coast of India. Curr. Sci. 2003; 86: 593-597.

Egorov NS. Antibiotics, a scientific approach. Mir Publishers, Moscow Farnet CM, Zazopoulos E. Improving drug discovery form microorganisms. In Natural products: Drug discovery and Therapeutic medicine. In: Zhang L, Demain AL, Editors. Totowa: Humana press inc, 1985. p. 95; 2005.

Parthasarathi S, Sathya S, Bupesh G, Durai Samy, et al. Isolation and Characterization of Antimicrobial Compound from Marine Streptomyces hygroscopicus BDUS 49. World J .Fish Marine Sci. 2012; 4 (3): 268-277,

Westley, JW, Evans RH, Sello LH et al. Isolation and characterization of antibiotic X- 14547A, a novel monocarboxylic acid ionophore produced by Streptomyces antibioticus NRRL, 8167. J. Antibiot. 1979; 32 : 100-107.

Pandey B, Ghimire P, Agrawal VP. Studies on the antibacterial activity of actinomycetes isolated from the Khumbu region of Mt.Everest. A paper presented in the International Conference on the Great Himalayas: Climate, Health, Ecology, Management and Conservation, Kathmandu. January12–15. Organized by KathmanduUniversity and the Aquatic Ecosystem Health and Management Society, Canada. 2004.

Kekuda TRP, Shobha KS, Onkarappa R. Studies on antioxidant and anthelmintic activity of two Streptomyces species isolated from Western Ghat soils of Agumbe, Karnataka. Journal of Pharm. Res. 2010; 3:26-29.

Yuan L, Zhang Y, Yu L et al. Actinopolymorpha Cephalotaxi sp. nov., a novel actinomycete isolated from rhizosphere soil of the plant Cephalotaxus fortune. Int J Syst Evol Microbiol. 2010; 60: 51-54.

Karsten GR, Drake HI. Comparative assessment of the aerobic and anaerobic microfloras of earthworm guts and forest soils. Appl Environ Microbiology. 1995; 61: 1039 – 1044.

Selvameenal L, Radakrishnan M, Balagurunathan R. Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening. Indian J Pharm Sci. 2009; 71: 499-504.

Oskay M, Same A, Azeri C. Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. Afr J Biotechol. 2004; 3:441-6.

Krishna SR, Sathish Kumar SR, Meenambekha L, Madhusudhan M. Antibacterial activity and characterisation of Actinobacteria isolated from Marine Bivalve Meretrix casta (Gmelin). Adv. in App Sci Res. 2011; 2(4): 431-439.

Dharmaraj S. Marine Streptomyces as a novel source of bioactive substances. World J Microb Biot. 2010; 26: 2123-2139.

Oskay M. Antifungal and antibacterial compounds from Streptomyces strains. Afr J Biotechnol. 2009; 8: 3007-3017.


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