Up-Regulation of TNF-α by Ethanol Extract of Moringa Oleifera in Benzene-Induced Leukemic Wister Rat: A Possible Mechanism of Anticancer Property

Authors

  • Adebayo Lawrence Adedeji Ladoke Akintola University of Technology
  • E.O. Akanni Biochemistry Department, College of Health Sciences, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Nigeria
  • R. A. Akanni Medical Microbiology & Parasitology Department, College of Health Sciences, Ladoke Akintola University of Technology, P.M.B. 4400, Osogbo, Nigeria
  • J. K. Oloke Pure & Applied Biology Department, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Nigeria

DOI:

https://doi.org/10.37591/rrjooh.v3i3.1356

Abstract

Ethanol extract of Monringa oleifera (EMO) has been shown to possess anti-cancer properties in animal models. The mechanisms of action of its anticancer properties have not been fully demonstrated. We induced leukemia in two groups of seven rats each by intravenous injection of benzene chromasolv solution (0.2 mL) 48 hourly for 4 weeks. EMO (0.2 ml of 100 mg/mL) was administered orally by gavage, after leukemia induction, daily for 4 weeks to one of the groups while the other group was not treated. The third group served as the normal control. At the end of the treatment, the rats were immobilized by cervical dislocation and blood samples were collected by cardiac puncture. Plasma concentrations of Beta-2 Microglobulin (β2M), Perforins (PF), Interleukin-2 (IL2), Interferon-gamma (IFN-γ) and Tumour Necrosis Factor-alpha
(TNF-α) were determined by standard commercial ELISA. Results showed that leukemia was induced within 4 weeks as significantly elevated leukocyte count and plasma β2M concentrations over the baseline were noted (p < 0.05). Plasma IL2 and TNF-α were also significantly elevated while IFN-γ decreased significantly in EMO treated rats compared with untreated groups. The plasma concentration of PF remained statistically unchanged. Since IL2 and PF are associated with cytotoxicity of CD8 T lymphocytes, reduced expression of PF in the face of increasing plasma concentration of IL-2 suggests that EMO may not exert its anticancer properties via direct cytotoxicity. We thus concluded that increased expression TNF-α would translate to enhanced apoptosis and may be a possible mechanism of action.

 

Keywords: Moringa oleifera, anticancer mechanism, cytokines

 

Downloads

Published

2019-04-10

Issue

Vol. 3 No. 3 (2014)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.