Arsenic Induced Cytotoxicity Study Against Human Epithelial Carcinoma (HeLa) Cell

Authors

  • Md. Shariful Islam Department of Animal Husbandry and Veterinary Science, Faculty of Agriculture, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Alam Khan Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh Department of Metabolism and Aging, The Scripps Research Institute, Florida-33458, USA
  • Hrithita Aftab Department of Material Science and Engineering, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Yoshifumi Kusumoto International University of Kagoshima, 8-31-1 Seiryo, Kagoshima 890-0032, Japan

DOI:

https://doi.org/10.37591/rrjot.v5i1.1313

Abstract

The present investigation was undertaken to find out the cytotoxic effects of various amounts of arsenic exposure to human epithelial carcinoma (HeLa) cell. Cells were treated with a final concentration (0, 10, 20, 30, 40 and 50 µg/l) of arsenite in 5 ml MEM for 24 h where 0 concentration was considered as control. Cell viability was determined by the trypan blue (TB) exclusion test using hematocytometer and automated BIO-RAD TC 10TM cell counting machine. It was demonstrated that survival rate of cancer cells treated with arsenite (0, 10, 20, 30, 40 and 50 µg/l) for 24 h decreased in a dose-dependent manner. Further, it was revealed that arsenite caused a time-dependent increase in cell death. In the highest concentration of arsenite (50 µg/l) cell survival rate was 1% and 2% in manual and automated counting. The possible mechanism that promote cytotoxicity of arsenite was addressed as oxidative damage of cells.

 

Keywords: Arsenite, HeLa Cells, Toxicity, Cell viability, Trypan blue assay, Hematocytometer

Cite this Article

Islam S, Khan A,Aftab H, Kusumoto Y. Arsenic Induced Cytotoxicity Study against Human Epithelial Carcinoma (HeLa) Cell. Research and Reviews: A Journal of Toxicology (RRJoT). 2015; 5(1): 1–8p.

Downloads

Published

2019-04-08

Issue

Vol. 5 No. 1 (2015)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.