Evaluation of Marine Waste Water And Aromatic Hydrocarbons Toxicity Using A Battery of Assays

Authors

  • Bright Obidinma Uba PhD, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, P.M.B.02 Uli, Anambra State, Nigeria
  • Edna Ifeoma Chukwura Professor, Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, P.M.B. 5025 Awka, Anambra State, Nigeria
  • Ikechukwu Harmony I heukwumere PhD, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, P.M.B.02 Uli, Anambra State, Nigeria
  • John Joseph Okeke Professor, Bio-conservation and Management, Nnamdi Azikiwe University, P.M.B. 5025, Awka, Anambra State, Nigeria
  • Ifeanyi Patience Akaun Lecturer, Department of General Studies, Delta State Polytechnic, Ogwashi-Uku, Delta State, Nigeria

DOI:

https://doi.org/10.37591/rrjot.v10i2.2099

Abstract

Abstract

The aim of this study is to evaluate the toxic effects of marine wastewater and representative aromatic hydrocarbons using a battery of assays. The methods employed for the toxicological evaluation involve physic-chemical analysis of the marine wastewater, toxi-chromo test using mutant E coli and sub-chronic animal toxicity testing with emphasis on body weight determination, biochemical indices and histopathological examination. The results showed that Nembe water side wastewater sample contain higher fractions of physic-chemical parameters than other sampled locations. Toxi-chromo testing revealed that the three sampling sites and aromatic hydrocarbons solutions were categorized in the order of toxicity: Onne wastewater > Nembe wastewater > Abonema wastewater and pyrene distilled water > anthracene distilled water > xylene distilled water with EC100 values of 0.28%, 0.35%, 0.40% and 0.22%, 0.30% and 6.44%, respectively. The mice model revealed no significant (P>0.05) changes in the animal weight, but extreme significant differences (P<0.05) were detected in the biochemical indices of the different doses of the aromatic hydrocarbons and their controls; with minor non adverse adaptive morphological changes in the observed tissues. These observations demonstrated the environmental and animal toxic effects of these test samples and strict regulations should be imposed on the indiscriminate discharge of these wastes to the environment.

Keywords: Albino mice, aromatic hydrocarbons, biochemical indices, E. coli, marine waste water, toxicity

Cite this Article

Bright Obidinma Uba, Edna Ifeoma Chukwura, Ikechukwu Harmony Iheukwumere, John Joseph Okeke, Ifeanyi Patience Akaun. Evaluation of marine waste water and aromatic hydrocarbons toxicity using a battery of assays. Research & Reviews: A Journal of Toxicology. 2020; 10(2): 1–13p.


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Published

2020-09-17

Issue

Vol. 10 No. 2 (2020)

Section

Research Article

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