HPLC and Tandem MS Device for the Recognition of Emtricitabine and Tenofovir Alafenamide in K2EDTA Human Plasma Utilizing Emtricitabine 15ND2 Tenofovir D5 as an IS

Authors

  • Arnav walia Associate Research Trainee, Bioanalytical Research, Synergen Bio Pvt. Ltd., Pune, Maharashtra, India.
  • Mansingh Rajput Head of Bio-analytical Research, Bioanalytical Research, Synergen Bio Pvt. Ltd., Pune, Maharashtra, India.
  • Neel Lahoti CEO, Synergen Bio Pvt. Ltd., Pune, Maharashtra, India
  • Vinayak Bhosale Senior Research Operator, Bioanalytical Research, Synergen Bio Pvt. Ltd., Pune, Maharashtra, India.
  • Arif Khan Senior Manager, Bioanalytical Research, Synergen Bio Pvt. Ltd., Pune, Maharashtra, India
  • Rishikesh Yadav Senior Research Associate, Bioanalytical Research, Synergen Bio Pvt. Ltd., Pune, Maharashtra, India.
  • Snehal Patil Research Associate-1, Bioanalytical Research, Synergen Bio Pvt. Ltd., Pune, Maharashtra, India.

Abstract

Developed very impressionable, discriminating, and exact extreme-efficiency liquid chromatography-tandem bulk spectrometry form for measurement of Emtricitabine and Tenofovir Alafenamide in human plasma. Emtricitabine15ND2 Tenofovir D5 was used as a within the standard (IS). The samples were divided on Phenomenex Kinetex C18 5 µm 100A, 100*4.6 mm procession utilizing Acetonitrile: 0.2% formic acid, 60:40 V/V as movable state, the retention period of Emtricitabine: 1.36 minutes (±0.5 minutes), Emtricitabine 15ND2: 1.36 minutes (±0.5 minutes), Tenofovir Alafenamide: 1.41 minutes (±0.5 minutes) and Tenofovir Alafenamide D5: 1.41 minutes (±0.5 minutes) individually. The precursor to product ion evolutions of Emtricitabine 130.200/248.200 m/z and Tenofovir 176.200/477.250 m/z were used to discover and compute Emtricitabine and Tenofovir Alafenamide and Emtricitabine 15ND2 Tenofovir D5 individually. A Shimadzu LC-MS-8045 whole-equipped accompanying vehicle propelled by ejection of pressurized gas or liquid ion spray (TIS) source and conducted in multiple reactions monitoring (MRM) style was pre-owned for the study.

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Published

2022-08-25

Issue

Vol. 12 No. 2 (2022)

Section

Research Article

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