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Reference of Circadian Rhythm in the Treatment of Psoriasis: A Novel Insight

G. V. Amruthavalli, Gayathri Rajagopal, Sri Ram .V

Abstract


The objective of the present study was to establish the needs for different products in the treatment of psoriasis with reference to circadian rhythm. The events like proliferation of keratinocytes and elastase activity are influenced by circadian rhythm. The proliferation of the keratinocytes occurs more during early hours of the day however, the enzymatic activities occur post to that.  Psorolin ointment and Psorolin oil were studied for the cell proliferation inhibition of the keratinocytes and elastase and LDH inhibition separately. In vitro assay on growth inhibition of keratinocytes by MTT, Cytotoxicity assay by measuring LDH, Tryphan blue exclusion assay, Elastase inhibition assay, Hyaluronidase inhibition assay. Findings of the study show that Psorolin oil inhibited the proliferation of keratinocytes in a non-cytotoxic manner as proved by lactate dehydrogenase (LDH) assay and Tryphan blue staining. The Psorolin ointment effectively inhibited elastase activity. Considering the time specificity cell division and enzymatic activity are separate, warrant the need of both Psorolin oil and Psorolin ointment for the treatment of psoriasis. In the present study confirm the need of two separate drugs viz., Psorolin oil and Psorolin ointment for the treatment  of psoriasis not due to their mechanism action was different but due to the effect of circadian rhythm in the pathogenesis of psoriasis.


Keywords


Circadian rhythm, Psoriasis, Cytotoxicity assay, Dr. JRK’s, Psorolin

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