Partial Characterization of Crude Lipase from Mutant Soil Fungal Isolate

Authors

  • Shreya .
  • Arun Kumar Sharma
  • Vinay Sharma
  • Jyoti Saxena

DOI:

https://doi.org/10.37591/rrjomv.v8i3.334

Abstract

Lipases are cluster of enzymes which cleaves oils and fats. Characterization properties of
lipases have been studied in the present investigation because they play an imperative role in
several industrial applications. The present lipase obtained from soil fungal strain named as
EMS5%-60min exhibited optimum activity at pH 7.0 (24.90±0.25 IU/ml/min) and 37ºC (20.28±0.51
IU/ml/min). In pH stability profile, the enzyme was found stable in alkaline pH range with
highest activity (13.08±0.07 IU/ml/min) at pH 9.0. Lipase demonstrated considerable activity
within the temperature range of 28ºC to 37ºC. The residual lipase activity after pre incubation
for 5 h with acetone was 194.09% (27.93±0.81 IU/ml/min) as compared to 100% activity
(14.39±0.21 IU/ml/min) of control. Similarly MgSO4, MnCl2, CaCl2, FeCl3, KCl and NaCl
increased lipase activity by 277.48%, 195.89%, 178.04%, 177.48%, 176.02% and 110.63%,
respectively as compared to control. H2O2 acted as oxidizing agent as it increased the activity
from 100% (14.39±0.21 IU/ml/min) to 201.52% (29±0.35 IU/ml/min). β- mercaptoethanol
drastically decline activity to 6.94% (1±0.84 IU/ml/min). Ariel (43.90%) and Patanjali
(24.84%) detergents showed less inhibition on activity, therefore present lipase can be used for
laundry process. The present lipase was found stable in presence of Tween-80 and xylitol.
Keywords: Lipases, characterization, solvents, surfactants, EMS (ethyl methane sulfonate)

Cite this Article

Shreya, Arun Kumar Sharma, Vinay Sharma et al. Partial Characterization of Crude Lipase from Mutant Soil Fungal Isolate. Research & Reviews: A Journal of Microbiology and Virology. 2018; 8(3): 79–89p.

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Published

2019-01-02

Issue

Vol. 8 No. 3 (2018)

Section

Research Article

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