Gossypetin Inhibits ROS Generation in HeLa and HepG2 Cell Lines

Abstract

Antioxidants are known for their ability to neutralize the reactive oxygen species present within the living system. These antioxidants are mainly secondary metabolites present in plant products and are generally known as nutraceuticals or bioactive compounds. Antioxidant can be categorized into many categories based upon their structure and activities like flavonoids, anthrocyanins and carotenoids. Flavonoids are heterogeneous polyphenolic compounds having beneficiary effect on human body and are also known to counteract in conditions like atherosclerosis and other heart conditions. Gossypetin is a hexahydroxyflavone, isolated originally from Hibiscus species and is known to exhibit anti-atherosclerotic potential and anti-microbial properties. In the present study, we have analyzed the antioxidant property of Gossypetin. DPPH radical scavenging activity showed 50% reduction at 40 μM concentration of Gossypetin. Here as for the first time, we have studied the antioxidant activity of Gossypetin in HeLa and HepG₂ cell line which is found to inhibit total ROS to 50% at the concentration above 50 μM. Gossypetin did not elicit any cytotoxic effect in both HeLa and HepG₂ cell line in dose and time dependent manner as evident by no changes in the morphology of HeLa cell. Immunoblot analysis in Gossypetin treated HeLa cell revealed the higher expression of NFκB and reduction in the level of caspase-3 confirming its anti-oxidative property.

Keywords: Antioxidant, Gossypetin, ROS, HepG₂, HeLa, oxidative stress

Cite this Article

Jogendra Singh Pawar, Saad Mustafa, Ilora Ghosh. Gossypetin Inhibits ROS Generation in HeLa and HepG₂ Cell Lines. Research and Reviews: Journal of Toxicology. 2017; 7(1): 25–31p.