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Toxicity Assessment of Pendimethalin with Reference to Biochemical Alteration (Carbohydrate, Lipid) of Fresh Water Fish Channa punctatus (Bloch)

Danish Syed, Jayashree Dhote, Pritesh kothekar, Irfan Ahmad Parray


Pendimethalin, a weedicide is a potential toxic pollutant, adversely affecting the fauna of aquatic ecosystem. The Channa punctatus were exposed to pendimethalin to determine LC50 values for 24, 48, 72 and 96 h and to study the behavioral changes. The aim of present study was to assess the 96 hours acute toxicity level of pendimethalin. The LC50 was determined by Finnyl’s Probit analysis. According to graphical plots, the 50% mortality values of pendimethalin for 96 h were 1.16 ml/l. These fishes were subjected to sub-lethal concentrations (1/2nd is 0.58 ml/l) of pendimethalin as per suggestions for different exposure periods (24, 48, 72, 96 hours). So, once the exposure of pendimethalin, the carbohydrate, lipid content in muscle, liver, gill, and the excretory organ was found to be deteriorated rapidly. Therefore, the hasty decrease in the content will interrupt the overall metabolism of the associate organism which may prove fatal.


Pendimethalin, Channa punctatus, carbohydrate, lipid

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Deka S, Mahanta R. Dichlorvos toxicity on fish – a review. Europ J Biol Res. 2015; 5(3): 78–85.

Tripathi G, Singh H. Impact of alphamethrin on biochemical parameters of Channa punctatus. J Environ Biol. 2013; 34(2): 227–230

Banaee M, Sureda A, Miravagefei AR,Ahmadi K. Histopathological alterations induced by diazinon in rainbow trout, Oncorhynchusmykiss. Int Environ Res. 2013; 7(3): 735–744. Article in Press.

Mellish S. Acute toxicity hazard of pesticides to freshwater fish. An article published by Agri-Environmental Specialist, PEI Department of Agriculture and Forestry; 2013.

Smith AE, Aubin AJ, McIntosh TC. Field persistence studies with emulsifiable concentrates and granular formulations of the herbicide pendimethalin in Saskatchewan. J Agri Food Chem. 1995; 43(11): 2988–2991.

Lee YD, Kim HJ, Chung JB,Jeong BR. Loss of pendimethalin in runoff and leaching from turfgrass land under simulated rainfall. J Agric Food Chem. 2000; 48(11): 5376–5382.

Engebretson J, Hall G, Hengel M,Shibamoto T. Analysis of pendimethalin residues in fruit, nuts, vegetables, grass, and mint by gas chromatography. J Agric Food Chem. 2001; 49(5): 2198–2206.

Miranda AL, Roche H, Randi MA, Menezes ML, Ribeiro CA. Bio-accumulation of chlorinated pesticides and PCBs in the tropical freshwater fish Hopliasmalabaricus: histopathological, physiological, and immunological findings. Environ Int. 2008; 34(7): 939–949.

Danion M, Le Floch S, Kanan R, Lamour F, Quentel C. Effects of in vivochronic exposure to pendimethalin/Prowl 400®on sanitary status and theimmune system in rainbow trout (Oncorhynchus mykiss). Sci Total Environ. 2012; 424: 143–152.

Schmidt Nielson B. Osmoregulation:Effect of Salinity and heavy metal. Fed Proc. 1975; 33(10): 2137–2146.

Muley DV, Karanjkar DM, Maske SV.Impact of industrial effluents 65 on the biochemical composition of Fresh water fish Labeorohita. J Environ Biol. 2007; 28(2): 245–249.

Remia KM, Logaswamy S, Logankumar K, Rajmohan D. Effect of an insecticide (Monocrotophos) on some biochemical constituents of the fish Tilapia mossambica. Poll Res.2008; 27(3): 523–526.

ShelkeAbhay D. Comparative study of cholesterol alteration in a freshwater teleost fish, Amblypharyngodonmola exposure to heavy metals. Bioscan J Life Sci. 2013; 8(3): 1001–1004.

Venkatramana GV, Sandhya Rani PN, Murthy PS. Impact of Malathion on the Biochemical Parameters of Gobiid Fish, Glossogobiusguiris (Ham). J Environ Biol. 2006; 27(1): 119–122.

Kalra, P., Sharma, S., Suman and Kumar, S. 2011. Antiulcer effect of the methanolic extract of Tamarindus indica seeds in different experimental models. J. Pharm. Bioallied Sci. 3(2): 236-341.

Frings C.S, Fendley T.W., Dunn R.T and Queen C.A., 1972. Improved determination of total serum lipids by the sulphovanillin reagent. Clin Chem., 18:673-674.


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